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The transmitted probe light was despatched to a fiber-coupled CCD-based spectrometer (QE65000, Ocean Optics), which captured and stored whole absorption spectra of 100-ms length at a frame fee of 10 s−1. SB-2358 Acton spectrograph, Princeton Instruments), able of recording a hundred ms per spectra at a frame price of 7.9 s−1. However, we could identify precisely the timing from the stop of the 488-pulse to the starting of the initially and subsequent details frames from laser fiducials showing up in each individual frame. X-ray diffraction data have been gathered at a hundred K at the LRL-CAT beamline (31-ID) of the Advanced Photon Source. Samples of R-GECO-1 or RCaMP1h were diluted in the microcuvet that contains buffer (MOPS/KCl/10mM-EGTA, pH 7.25), and info taken for this "no-calcium" situation. 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